s epidermidis rp62a atcc35984 rp62a strain  (ATCC)

Journal: bioRxiv

Article Title: Biophysical properties and phenotypes of cell clusters detached from Staphylococcus epidermidis biofilms after matrix-targeted disruption

doi: 10.64898/2026.01.28.701379

Figure Lengend Snippet: Average physical properties of cells and cell clusters detached from S. epidermidis biofilms after matrix-targeted biofilm disruption. (A) Average concentration of cells detached from biofilms. Statistical significance determined by one-way ANOVA with Dunnett’s T3 multiple comparisons tests (**P ≤ 0.01). (B-E) Average (B) number of cells per cluster, (C) Euclidian diameter, (D) anisotropy index, and (E) asphericity of cell clusters detached from S. epidermidis RP62A biofilms with no treatment or treated with NaIO 4 , Proteinase K, DNase I, or pH 10 TSB G with statistical significance determined by non-parametric Kruskal-Wallis tests with Dunn’s multiple comparison tests (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).

Article Snippet: S. epidermidis RP62A (ATCC 35984), a PIA-dependent biofilm forming strain was used as the primary model organism.

Techniques: Disruption, Concentration Assay, Comparison

Journal: bioRxiv

Article Title: Biophysical properties and phenotypes of cell clusters detached from Staphylococcus epidermidis biofilms after matrix-targeted disruption

doi: 10.64898/2026.01.28.701379

Figure Lengend Snippet: k- means clustering of the biophysical properties of cellular clusters detached from S. epidermidis RP62A biofilms. (A) Two-dimensional t-distributed stochastic neighbor embedding (t-SNE) visualization of the five distinct biofilm detached cell phenotypes. (B) Projections of 3D CLSM images (left column) and renderings (right column) of representative bacterial cell clusters for each biofilm-detached cell cluster phenotype. Scale bars = 2 µm. Phenotype 1 (purple circle) contains small, oblate clusters, Phenotype 2 (blue square) contains mid-sized, oblate clusters, Phenotype 3 (green inverse triangle) contains large, oblate clusters, Phenotype 4 (orange triangle) contains small, spherical clusters, and Phenotype 5 (pink diamond) contains small, prolate clusters.

Article Snippet: S. epidermidis RP62A (ATCC 35984), a PIA-dependent biofilm forming strain was used as the primary model organism.

Techniques:

Journal: bioRxiv

Article Title: Biophysical properties and phenotypes of cell clusters detached from Staphylococcus epidermidis biofilms after matrix-targeted disruption

doi: 10.64898/2026.01.28.701379

Figure Lengend Snippet:

Article Snippet: S. epidermidis RP62A (ATCC 35984), a PIA-dependent biofilm forming strain was used as the primary model organism.

Techniques:

Journal: bioRxiv

Article Title: Biophysical properties and phenotypes of cell clusters detached from Staphylococcus epidermidis biofilms after matrix-targeted disruption

doi: 10.64898/2026.01.28.701379

Figure Lengend Snippet: Relative abundance of S. epidermidis biofilm detached cells after matrix-targeted biofilm disruption across different growth conditions and strains. Relative abundance of biofilm detached cells, where the concentration or absorbance (OD 600 ) of cells detached from biofilms after treatment is normalized to the untreated condition, for: (A) S. epidermidis RP62A biofilms grown in flow at a shear stress of 0.1 Pa or statically in a 96-well plate, and (B) eight S. epidermidis strains: RP62A, 1457, P2, P6, P12, P18, P37 and P47 grown in a 96-well plate. Statistical significance is determined by two-way ANOVA with Dunnett’s T3 multiple comparison tests (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).

Article Snippet: S. epidermidis RP62A (ATCC 35984), a PIA-dependent biofilm forming strain was used as the primary model organism.

Techniques: Disruption, Concentration Assay, Shear, Comparison